| 来源类型 | Article |
| 规范类型 | 其他 |
| DOI | 10.1016/j.bjm.2017.01.001 |
| Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276. | |
| Furlan V; Batista I; Bandarra NA | |
| 发表日期 | 2017 |
| 出处 | Brazilian Journal of Microbiology 48 (2): 359-365 |
| 出版年 | 2017 |
| 语种 | 英语 |
| 摘要 | The high costs and environmental concerns associated with using marine resources as sources of oils rich in polyunsaturated fatty acids have prompted searches for alternative sources of such oils. Some microorganisms, among them members of the genus Aurantiochytrium, can synthesize large amounts of these biocompounds. However, various parameters that affect the polyunsaturated fatty acids production of these organisms, such as the carbon and nitrogen sources supplied during their cultivation, require further elucidation. The objective of this investigation was to study the effect of different concentrations of carbon and total nitrogen on the production of polyunsaturated fatty acids, particularly docosahexaenoic acid, by Aurantiochytrium sp. ATCC PRA-276. We performed batch system experiments using an initial glucose concentration of 30 g/L and three different concentrations of total nitrogen, including 3.0, 0.44, and 0.22 g/L, and fed-batch system experiments in which 0.14 g/L of glucose and 0.0014 g/L of total nitrogen were supplied hourly. To assess the effects of these different treatments, we determined the biomass, glucose, total nitrogen and polyunsaturated fatty acids concentration. The maximum cell concentration (23.9 g/L) was obtained after 96 h of cultivation in the batch system using initial concentrations of 0.22 g/L total nitrogen and 30 g/L glucose. Under these conditions, we observed the highest level of polyunsaturated fatty acids production (3.6 g/L), with docosahexaenoic acid and docosapentaenoic acid ω6 concentrations reaching 2.54 and 0.80 g/L, respectively. |
| 主题 | Ecosystems Services and Management (ESM) |
| 关键词 | Carbon source Docosahexaenoic acid Nitrogen source Polyunsaturated fatty acids Thraustochytrids |
| URL | http://pure.iiasa.ac.at/id/eprint/14373/ |
| 来源智库 | International Institute for Applied Systems Analysis (Austria) |
| 引用统计 | |
| 资源类型 | 智库出版物 |
| 条目标识符 | http://119.78.100.153/handle/2XGU8XDN/130820 |
| 推荐引用方式 GB/T 7714 | Furlan V,Batista I,Bandarra NA. Production of docosahexaenoic acid by Aurantiochytrium sp. ATCC PRA-276.. 2017. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 资源类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 1-s2.0-S151783821730（986KB） | 智库出版物 | 限制开放 | CC BY-NC-SA | 浏览 | ||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
