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Conservation Trust Funds (CTFs) are a source of sustainable financing for long-term biodiversity conservation, in particular for protected areas management. Through a review of 12 case studies from Africa, Asia, Latin America, the Caribbean, and Australasia, this research report provides a broad overview of how to create a CTF, describing its legal and institutional structure, fund generation and delivery, and identifying when it might be an appropriate tool. The lessons learnt from the case studies provide guidance on best practice and an insight into the conditions for the sustainability and success of the funds, and thereby their value to conservation.